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KMID : 0380420110350010007
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Journal of Prventive Veterinary Medicine
2011 Volume.35 No. 1 p.7 ~ p.12
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Distribution of serotype and biofilm of Actinobacillus pleuropneumoniae isolated from pigs
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Shin Dong-Ho
Byun Jae-Won
Kim Ha-Young
Kim Dae-Keun
Jang Won-Nam
Moon Oun-Kyoung
Lee O-Soo
Jung Byeong-Yeal
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Abstract
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The present study was conducted to investigate serotype distribution and biofilm formation of Actinobacillus (A.) pleuropneumoniae isolated from pneumonic lungs of pigs. A total of 37 A. pleuropneumoniae were isolated between January 2009 and June 2010. Serotypes of A. pleuropneumoniae isolates were determined using two different PCRs. The majority of isolates belonged to serotype 5 (n=31, 83.8%), and the others belonged to serotype 1 (n=4, 10.8%), and 2 (n=2, 5.4%), respectively. The ability of biofilm formation of the isolates was also determined by quantitative microtiter plate assay. Biofilm formation was observed in both 23 (62.2%) of the 37 field isolates and seven (43.8%) of the 16 reference strains. On the other hand, biofilm formation was various according to the serotypes: 20 (64.5%) of serotype 5, and three (75.0%) of serotype 1. However, two isolates of serotype 2 did not produce the biofilm in this study. Consequently, A. pleuropneumoniae serotype 5 was the most frequently detected (83.8% of the isolates), and 23 (62.2%) of 37 isolates exhibited biofilm positive phenotype in this study.
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KEYWORD
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Actinobacillus pleuropneumoniae, Biofilm, PCR, Pig, Serotype
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